A radioimmunoassay for rat serum parathyroid hormone using N-terminal of PTH.
نویسندگان
چکیده
Partially purified bovine parathyroid hormone (PTH) produced two kinds of antisera, GP-100 reacting with 125I-labeled bovine PTH (b-PTH 1-84) alone and GP-M reacting with 125I-labeled synthetic N-terminal portion of bovine PTH (b-PTH 1-34) as well as b-PTH 1-84. Rat serum PTH reacted with the latter but not in the former, suggesting a similarity to b-PTH 1-34. Rat serum PTH became undetectable after parathyroidectomy, and increased after Na2-EDTA injection and following renal injury with sodium sulfacetylthiazole.
منابع مشابه
Radioimmunoassay specific for amino (N) and carboxyl (C) terminal portion of parathyroid hormone.
A radioimmunoassay specific for the amino (N) terminal portion of the parathyroid hormone (PTH) molecule (N-PTH radioimmunoassay) has been developed by iodinating synthetic 1-34bovine PTH (1-34bPTH) and using commercially available bPTH antiserum. A radioimmunoassay specific for the carboxyl (C) terminal (C-PTH radioimmunoassay) has been carried out by adding enough amount of 1-34bPTH to the PT...
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Highly purified bovine parathyroid hormone (b-PTH 1-84) and its synthetic N-terminal peptide (b-PTH 1-34) were labelled with 125-I and incubated with rat kidney homogenate at 37 degrees C for 1 hour to assess the degree of hydrolysis of the iodinated peptides through measurement of the increase of trichloroacetic acid soluble 125-I fraction. Rat kidney homogenate rapidly hydrolyzed b-PTH 1-84 b...
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The role of various parathyroid hormone (PTH) radio-immunoassays in the diagnosis of primary hyperparathyroidism (PHP) is controversial. A series of 204 patients with surgically proven PHP was studied. Serum total calcium, serum ionized calcium, amino (N)-terminal PTH and carboxyl(C)-terminal PTH were assessed in relation to the volume and weight of adenomatous or hyperplastic parathyroid tissu...
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ورودعنوان ژورنال:
- Endocrinologia japonica
دوره 22 2 شماره
صفحات -
تاریخ انتشار 1975